Conditioned medium (RPMI 1640) from ConA-stimulated rat splenocyte cultures containing 10% fetal bovine serum and 10mM HEPES
Medium with ConA supports high-titer HIV production by leukocytes
Medium without ConA (from washed rat splenocyte cultures) designed for applications that support proliferation of lectin-activated T-cells and single-cell cloning of antigen-specific T-cells from polyclonally activated primary cultures
Both undergo an appropriate bioassay, are filtered through a 0.2µm membrane and are tested for the absence of bacteria, fungi and Mycoplasma—guaranteeing a high-quality product
